Glucose Concentration In Drinks Assignment Sample

Glucose Concentration In Drinks

Abstract

The entire purpose of the glucose experiment is to find out the concentration of the glucose in the two given drink. Absorbance difference of the given drinks has been used for the determination of the glucose concentration. Entire results of the experiments had been organized logically. Throughout the entire report glucose concentration in Drink 1 (Orange juice) is about 0.025 per 100 ml. Presence of glucose concentration in Drink 2 (Rockstar) is 0.018 per 100 ml. There are stacks of establishment issues have been perceived. Investigate results has gotten a handle on close by the combination of glucose. There are a several experimental results have been included throughout the entire calculation. Several problems have been identified through the entire experiment. There are lots of background issues have been identified. Experiment results has explained along with the concentration of glucose. There are several Concentration measurement results have included within the calculation. Entire outcomes of the assessments had been facilitated honestly. A couple of issues have been recognized through the entire preliminary.

Introduction

Initially a preparation of the essential reagent has followed several standarity level along with the water. From 0 standarity level along with the water of 700μL. Final standarity level was about the drink 1 that contains 665μL of water. Several experiment step are produced for Glucose assay reagent. A graph has been plot based on the difference of Absorbance (A2-A1) vs the concentration of glucose (Ebrahimi et al. 2020). Lots of experiments results are there. There were several key components that had been noted based on the absorbance of Sucrose (A2-A1) along with the p.H and temperature. There is a pre lab table that has been fulfilled with the various measurement. There are lots of materials are used along with the appropriate method.

Reaction Sequence:

D-glucose + ATP + (Enzyme: Hexokinase) → Glucose -6-phosphate + ADP

Glucose -6-phosphate + NADP⁺ + (Enzyme: Glucose-6-phosphate dehydrogenase) → 6-phosphogluconate + NADPH + H⁺

In the determination of Glucose concentration in the drinks, there were several inaccuracies that had a huge impact in the measurement as well as in the process of calibration. Inaccuracy in the measurement technique including pipetting as well as mixing. Temperature measurement was another problem during the entire experiment. The entire objective of the experiment is to find out the concentration measurement of glucose in the drink. There were several issues in the background of glucose especially in the field of sports drink (Kandwal et al. 2020). In the time of physical effort, there will be a lot of energy requirements of energy in the muscles. It helps to maintain the level of blood sugar. Glucose concentration within sports drinks helps to balance the issues of digestion along with the crashes of energy.

Materials and Method

Several methods have been used throughout the entire experiment. First method that has been used is about the method of reducing. After that the next method that has been used is about the method of concentration. Next method has been used is the enzymatic method (Morabito et al. 2020). The figure is showing the several components including the solution of glucose with the volume 20 mM along with H2O. 1 mL of standard glucose solution to be prepared in every tube. Dilution had been calculated by using the following formula; ‘C1V1=C2V2’.

Table 1: Pre Lab

Results and Calculations

Figure 1: Absorbance difference (A2-A1) v/s Concentration of Glucose

(Source: Self-created)

Experiment Results

Table 2: Experiment Results

Preparation of the Reagent

For the preparation of the reagent several process had followed, mentioned below;

For the standarity at 0, 700 μL of water had been added. For the standarity at 1, 35 μL of 1G had been added along with 665 μL of water. For the standarity at 2, 35 μL of 2G had been added along with 665 μL of water. For the standarity at 3, 35 μL of 3G had been added along with 665 μL of water. For the standarity at 4, 35 μL of 4G had been added along with 665 μL of water. For the standarity at 6, 35 μL of 6G had been added along with 665 μL of water (Luna et al. 2021).

Discussion and Conclusions

Theory Comparison

Glucose assay reagent and Experiment Steps

300 μL of glucose assay reagent solution had been added in each of the cuvette labeled.

Experiment steps

Several steps have been followed for the entire experiment. In the very first stage at the labeled value of 0, 300μL of reagent had been added. Then the entire solution mixed well. After that absorbance had been recorded at the value of 340 nm (Haque et al.  2021). After starting the timer, step 1 has been repeated with various timing separation such as 2 mins, 4 mins, 6 mins, 8 mins, 10 mins, 12 mins, 14 mins and 18 mins for the several cuvettes labeled values of Drink 1, Drink 2 along with another cuvettes labeled such as 1,2,3,4 and 6 respectively. At 0 standard labeled, 700 μL of water has been added with the 300 μL of the assay reagent of glucose. At the standard label of 1, 35 μL of 1G has been added with the 665 μL of water along with 300 μL of the assay reagent of glucose. At Standard labeled 2, 35 μL of 2G tube has been added with 665 μL of water along with the assay reagent of glucose. At the standard label of 3, 35 μL of 3G has been added with the 665 μL of water along with 300 μL of the assay reagent of glucose. At the standard label of 4, 35 μL of 4G has been added with the 665 μL of water along with 300 μL of the assay reagent of glucose. At the standard label of 6, 35 μL of 6G has been added with the 665 μL of water along with 300 μL of the assay reagent of glucose. For the measurement of Drink 1, 35 μL of drink 1 has been added with 665 μL of water along with the 300 μL of assay reagent of glucose. For measurement of drink 2, 665 μL of water along with the 300 μL of assay reagent of glucose. For sucrose, 665 μL of water along with the 300 μL of assay reagent of glucose.

Discuss and Significance of the result 

For calculation of glucose concentration, “V1C1=V2C2” formula has been used whereas V1 refers to the stock solution volume (Mannino et al. 2021). V1 refers to the stock solution concentration, V2 refers to the final volume of the diluted solution and C2 refers to the final concentration of the dilute solution. By using the reducing method, properties of glucose solution have been reduced along with changes in metal states. Assay reagent of glucose has been produced throughout the several experimental steps. 

For Drink 1 (orange juice), calculation of glucose concentration on the D1 = 1 * 0.25 + 0 = 0.25. Now glucose concentration on Drink 1 = 0.25 * 10/100 = 0.025/100 ml. For Drink 2 (Rockstar) glucose concentration D2 = 1 * 0.18 ml. Now presence of glucose concentration in Drink 2 per 100 ml is about = 0.18 * 10/100 = 0.018/ 100 ml. There are several steps that could be followed for improving the method of concentration measurement of glucose within the laboratory. First component to be followed is about the control of quality along with the calibration. By calibrating the equipment of the spectrometer and implementation of quality control could help in the improvement of the measurement quality. After that the next important component is about the protocol of standardization. During the preparation of the sample, standardization of the protocol is very important. Usage of standard material along with the material is another component. After that the next component is about studies of validation along with the control of the environment. Apart from these, there are several components including, maintenance of the instruments, technical analysis of the data, overall improvement etc.

By the use of protocol standardization during the preparation of the sample along with the analysis minimize several variations in the results. After that using standard material along with the reference, there are several incorporated materials that are used in the concentration measurement of glucose. Appropriate reference for material helps to increase the overall accuracy in the results of concentration measurement. In the measurement (Archana et al. 2020). By the use of data analysis techniques, there are several advance level implementations of this method including the overall performance along with the significant adjustment.

There are a couple of stages that could be followed for dealing with the strategy for obsession assessment of glucose inside the lab. Initial segment to be followed is about the control of significant worth close by the change. By changing the equipment of the spectrometer and execution of significant worth control could help in the improvement of the assessment quality. After that the accompanying huge part is about the show of standardization. During the availability of the model, standardization of the show is essential. Usage of standard material close to the material is another part. After that the accompanying part is about examinations of endorsement close by the control of the environment. Beside these, there are a couple of parts including, backing of the instruments, specific examination of the data, as a rule, etc. By the usage of show standardization during the status of the model close by as far as possible a couple of assortments in the results. After that using standard material close by the reference, there are a couple of combined materials that are used in the obsession assessment of glucose. Reasonable reference for material helps with growing the overall precision in the results of obsession assessment. In the assessment. By the use of data assessment strategies, there are a couple of improvement level executions of this procedure including the overall show close to the basic change.